A CYTOTOXIC ANALYSIS OF A SARDINIAN PLANT EXTRACT CREAM ON HUMAN ORAL PRIMARY CELL CULTURES: AN IN VITRO STUDY

Cellular Concentration and Response: In-depth Analysis on HPDLSC and HG

The experimental protocol involved incubating cells at different concentrations of RD7 (0.5, 1, 2.5, and 5 mg/ml) to assess their response. Evaluation methodologies included colorimetric tests (MTT assay) and trypan blue exclusion tests for cell proliferation and viability, while optical and scanning electron microscopes provided information on morphological changes.

The results revealed a decrease in cell growth at high concentrations (2.5 mg/ml and 5 mg/ml) starting from 24 hours of incubation. This reduction was correlated with evident morphological changes, while lower concentrations (0.5 and 1 mg/ml) maintained a typical morphology. Furthermore, the wound healing test showed complete wound closure after 24 hours of treatment with low concentrations of RD7.

RD7: a promising clinical option for tissue regeneration

The analysis of the results suggests that low concentrations of RD7 not only do not show cytotoxic effects, but also stimulate cell proliferation and contribute to the induction of migratory properties in hPDLSC and hGF. These results pave the way to consider RD7 as a potential product to be incorporated into clinical practice to promote tissue regeneration. The innovative approach of this study provides a significant contribution to the understanding of the effects of Sardinian plant extracts on human cells, promoting research towards increasingly effective solutions in the field of regenerative medicine.